Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens

In this article the unnatural amino acid mutagenesis was used to investigate the acid-survival strategy and the pathogenesis mechanisms of some enteric bacterial species. They reported the incorporation of a photo-cross linking amino acid into a Shigella acid chaperone shHdeA and an amino acid bearing an azido group was incorporated into a Shigella type 3 secretion effector OspF. This is the first time that UAAs were utilized to investigate proteins involved in the pathogenesis and stress-defense mechanisms of virulent Gram-negative bacteria.