Choosing an effective protein bioconjugation strategy

As we recently discussed in class there are a wide variety of chemical reactions for protein bioconjugation. This review provides a decision tree that can be used to determine the correct bioconjugation reaction for the desired application. The picture is really big, so i'm no going to attach it to the post. I really like the use of logic here in designing a "map" to lead to the best bioconjugation based on the properties of the protein target. They cover a lot of the techniques we learned, such as native chemical ligation using cysteine, HaloTag, and biotin ligase. An example of use and application of the decision tree is presented.