This paper utilizes quarom sensing circuitry for the directed evolution of AHL-quenching enzymes. Acyl-homoserine lactones (AHL) are a class of diffusible molecules used in bacterial intercellular communication. It is expressed during periods of stress to a bacteria's host organism as an intercellular signal leading to increased virulences when the host's immune response system is weakened. The goal of the authors is to use directed evolution of AHL-quenching enzymes to control AHL concentrations. Through directed evolution two evolved enzymes are produced with increased kcat/kM values, due mainly to increased substrate sensitivity. The screening method used conferred antibiotic resistance to enzymes able to efficiently quench AHL molecules. This study may be used as a template for further quarom circuitry probing and the evolution of enzymes involved.
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