Probing essential nucleobase functional groups in aptamers and deoxyribozymes by nucleotide analog interference mapping of DNA

This paper shows a chemical method for structural probing of function within DNA molecules:

"Nucleotide analog interference mapping of DNA (dNAIM) is here introduced as a new non-enzymatic interference-based approach that enables high-throughput identification of essential nucleobase functional groups in DNA aptamers and in the catalytic core of deoxyribozymes. Nucleobase-modified ribonucleotides are statistically incorporated into DNA by solid-phase synthesis, employing the 2’-OH group as a chemical tag for analysis of interference effects."