This paper shows how various biomolecules such as nucleic acids and proteins can be fluorescently labeled via 'click' reaction. The benzothiazole alkyne is weakly fluorescent until it is 'clicked' onto an amino acid or nucleoside with an azide group to form the triazole, which greatly increases the fluorescent intensity so that it can be seen in cells. Since the alkyne is weakly fluorescent, the background fluorescence is greatly reduced.
Wednesday, August 31, 2011
Developing Visible Fluorogenic ‘Click-On’ Dyes for Cellular Imaging
This paper shows how various biomolecules such as nucleic acids and proteins can be fluorescently labeled via 'click' reaction. The benzothiazole alkyne is weakly fluorescent until it is 'clicked' onto an amino acid or nucleoside with an azide group to form the triazole, which greatly increases the fluorescent intensity so that it can be seen in cells. Since the alkyne is weakly fluorescent, the background fluorescence is greatly reduced.
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We got scooped here!!
ReplyDeleteIt unfortunately happened and should serve as a reminder that you are always competing with the rest of the scientific world to be the first to publish a new discovery or a new technology!