Intracellular Ca2+ levels play a crucial role in the control of ATP synthesis. However, the spatiotemporal correlation between ATP and Ca2+ remains unclear due to the inability to visualize these factors within same individual cells. In this paper a new red-shifted ATP probe called GO-ATeams was developed, in which green fluorescent protein (GFP)and orange fluorescent protein (OFP) were used as a FRET pair. This new probe allows for better resolution at the single cell level.
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