MMP-9 is a enzyme in the family of matrix metalloproteinases. It is believed to be responsible for invasion and metastasis of cancer cells. In this paper the MMP-9 transcript was probed with a complementary DNAzyme. This showed that the DNAzyme lowered the concentration of the MMp-9 transcript, which lowered the rate of invasion and metastasis of the cultured cancer cells.
Somewhat questionable results in this paper, since the high magnesium concentrations required for DNAzyme activity are typically not found in cells. They also show qRT PCR amplification curves in the main text which is quite unusual. The control oligo should also degrade the mRNA based on an RNaseH mechanism.
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