This paper describes RNA-based control modules that allow for the tuning of protein expression levels in yeast. The Rnt1p substrate library described can be used to predictably tune gene expression, in this case gene regulation, in concert with any promoter. Two design strategies were implemented. First, a 'clamp' region was added to the base of each Rnt1p hairpin. Second, only sequences that formed predicted hairpin structures at the lowest free energies were included within the Rnt1p control module set. This prevents any flanking sequences from disrupting the desired folding of the control modules which could vary function in a genetic context. The Rnt1p library provides a key tool for synthetic biology applications in yeast.
Great paper! Not only are these devices tunable, but they are also largely context independent - use them with any promoter and target gene!
ReplyDeleteI found their promiscuity towards any target gene to be an intriguing feature as well.
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