This paper discusses the T7 helicase enzyme, which unwinds DNA using dTTP. The authors found that adding ATP would also allow unwinding but caused the helicase to 'slip' along the DNA. Enzyme kinetics were also discussed to determine the effect of slippage frequency. Further discussion on the binding of the enzyme to DNA is also included.
Sunday, October 16, 2011
ATP-induced helicase slippage reveals highly coordinated subunits
This paper discusses the T7 helicase enzyme, which unwinds DNA using dTTP. The authors found that adding ATP would also allow unwinding but caused the helicase to 'slip' along the DNA. Enzyme kinetics were also discussed to determine the effect of slippage frequency. Further discussion on the binding of the enzyme to DNA is also included.
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