The authors of this paper have developed a method to detect synthetic lethal drug-gene interactions in tumor models. They introduce a single genetic modification into a normal cell line such as healthy breast cells. With that modification they also introduce a barcode , which is a short, nontranscribed stretch of DNA which can be selectively quantified by PCR. The number of PCR reads corresponds to how much of that barcode is present, which corresponds to numbers of cells carrying that particular genetic modification in a population of cells.
Thursday, October 20, 2011
A chemical-genetic screen reveals a mechanism of resistance to PI3K inhibitors in cancer
The authors of this paper have developed a method to detect synthetic lethal drug-gene interactions in tumor models. They introduce a single genetic modification into a normal cell line such as healthy breast cells. With that modification they also introduce a barcode , which is a short, nontranscribed stretch of DNA which can be selectively quantified by PCR. The number of PCR reads corresponds to how much of that barcode is present, which corresponds to numbers of cells carrying that particular genetic modification in a population of cells.
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