Sauer’s group engineered a split-adaptor system which split-adaptor proteins are functional when small molecule is present. The active adaptor then is able to deliver the protease recognized tagged-substrate to a ClpXP protease complex as a result the substrate is lysed. By adding a tag to the target protein this method can be used to study transcription and translational mechanism.
I'll mention in my presentation on Thursday a similar system of targeting proteins for degradation using a chemical inducer of dimerization (CID). This system also uses a CID, rapamycin, to induce dimerization of the split adaptor protein.
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