In this paper is presented a system for determining density of bacteria and fungi in order to determine susceptibility to antibiotics. It is faster than traditional methods, requiring only 4-5 hours as opposed to 24-48 hours. The basic concept is that the surface of the bacteria, which is negatively charged, breaks apart an interaction of negatively charged fluorescein (FL) with positively charged polyfluorene (PFP). Breaking apart this interaction disrupts FRET between FL and PFP, and a change in emission is seen.
Friday, September 30, 2011
Rapid, Simple, and High-Throughput Antimicrobial Susceptibility Testing and Antibiotics Screening
In this paper is presented a system for determining density of bacteria and fungi in order to determine susceptibility to antibiotics. It is faster than traditional methods, requiring only 4-5 hours as opposed to 24-48 hours. The basic concept is that the surface of the bacteria, which is negatively charged, breaks apart an interaction of negatively charged fluorescein (FL) with positively charged polyfluorene (PFP). Breaking apart this interaction disrupts FRET between FL and PFP, and a change in emission is seen.
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