The research presented in this paper uses caged aptamers as a way to detect analyte proteins, followed by elution under mild conditions and qPCR. The advantage of this technique over immuno-PCR, which employs antibodies attached to a short DNA strand, is that the aptamer both binds the target and is the template for quantification. Because light is used to free the analyte, no damage is done to either the aptamer or the target protein, so further analysis is possible.
No comments:
Post a Comment