Gene regulation is one of the foci of synthetic biology research. This paper provides further insight into the engineering of components of a gene regulatory network for whole-cell biosensors’ physical and chemical signals. Its aim is to increase the sensitivity of a particular genetic circuit towards XylR inducers. XylR is a transcriptional regulator of the TOL pathway of Pseudomonas putida mt-2. The TOL plasmid encodes a pathway for the catabolism of toluene and xylenes.
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